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sgRNA Synthesis

Levostar Biotech is your trusted partner from discovery to delivery in CRISPR-based therapy development, offering high-quality synthetic single guide RNA (sgRNA) using the latest technological advancements. Our sgRNA ensures superior CRISPR editing efficiency, reduced cytotoxicity, and consistent lot-to-lot stability, empowering researchers to achieve reliable and impactful results in their experiments. More importantly, we provide “HyperPure“ ultra-long pegRNA ranging from 150-400nt for prime editing and other next-generation editors.

Service Advantages

Extended Length

Synthesis of sgRNA and pegRNA up to 230nt

High Purity

>90% purity purificated by HPLC and confirmed by LC-MS QC

Versatile Modifications

Options for fluorescent labeling and other tailored enhancements

Flexible Design

Tailored sgRNA sequences for accurate gene targeting

Service Details

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Inquiry Input	● Length: Up to 230nt
	● Product Type: sgRNA or pegRNA
	● Modifications: 2'-O-Methyl Modification (m), Phosphorothioate Modification(*), customized modification, or not needed
Purification Method	● Method: Desalted or HPLC
Deliverables	● Quantity: Choose from 2nmol to 100nmol, lyophilized or liquid
	● Delivery format: Single tube or 96-well plates
Turnaround Time Options	＜110 nt	From 7 business days
	110-140 nt	From 5 weeks
	140-180 nt	From 7 weeks
	＞180 nt	TBD

Comment: Default m and * modifications are included at no charge. Other custom modifications are available upon request.

Sequences longer than 110 nt are purified by HPLC.

*To learn more about LNP encapsulation, please click here.

Resource

LNP Encapsulation

Learn about Levostar’s end-to-end LNP encapsulation platform, from sequence design and RNA production to scalable encapsulation, nanoparticle characterization, and final product delivery.

LNP Encapsulation

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LNP Encapsulation