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Gene Synthesis

The Levostar Biotech team has been dedicated to gene synthesis for over a decade, synthesizing millions of genes for clients worldwide. They specialize in complex gene sequences, including repeat sequences, high-GC content sequences, and ITR structures. Notably, for mRNA plasmid synthesis, they ensure precise control over the polyA tail length. Additionally, we provide comprehensive, one-stop solutions tailored to our customers' experimental needs, offering personalized design consultation, codon optimization, and vector construction recommendations.

Service Advantages

100%

Sequence Accuracy

>99%

Success Rate

>95%

On-Time Delivery

Fast Turnaround

Delivery in as little as 5 days

Vector Variety

Extensive library of commonly used vectors

Expertise in synthesizing challenging and large genes

Complex Gene Solutions

Expertise in challenging and large genes

End-to-End Production

From sequence to plasmid/antibody/mRNA

High-Quality Plasmids

>90% supercoiled plasmid

Service Details

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Sequence Input
	● Length: Up to 100,000 bp
	● Format: Nucleotide or amino acid sequences
	● Codon optimization: Expression host, restriction sites to avoid
Cloning Options
	● Vectors: Choose from Levostar vector library or customer vector
	● Cloning Methods: Choose from restriction cloning, or seamless cloning
Plasmid DNA Options
	● Quantity: Choose from 5µg to 100mg, lyophilized or liquid
	● Supercoil: Choose from predominantly supercoil, ≥ 80% or ≥ 90% supercoil
	● Endotoxin Level: Choose from ≤ 100EU/mg, ≤ 50EU/mg, ≤ 10EU/mg, or not guarantee
Turnaround Time Options	Rapid Synthesis	Standard Synthesis	Fragment Synthesis
	From 72 hours	From 5 business days	From 3 business days

Deliverables

● 5μg of lyophilized plasmid
● Certificate of analysis (electronic)
● Construct map for the plasmid (electronic)
● Sanger sequencing result covering the gene insert (electronic)
● Sequencing alignment files for the plasmid (electronic)
● Free onboarding and storage of submitted samples and final constructs for up to 2 years

Resource

Gene Synthesis

Learn about our comprehensive gene synthesis services, from sequence optimization and codon design to de novo DNA synthesis, cloning, sequence verification, and plasmid preparation, supporting your research from gene design to ready-to-use constructs.

Gene Synthesis

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Gene Synthesis