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LNP Encapsulation

Lipid Nanoparticles (LNPs), as a next-generation delivery platform, utilize lipid molecular self-assembly to create nano-scale encapsulation systems. This technology significantly enhances drug stability and targeting efficacy.

Levostar Biotech has established a fully integrated technology platform covering gene synthesis, plasmid preparation, mRNA synthesis, and LNP encapsulation, with breakthrough capabilities in efficient delivery of circular RNA (circRNA) and self-amplifying RNA (saRNA). We provide full-cycle solutions from sequence design to final product scale-up for innovative drug development

Service Advantages

·End-to-End Integration：From Sequence to LNP products
·Superior Nanoparticle Characterization：QC analytics ensures precision and reliability
·Flexible Scaling Capacity：Manufacturing from 100 μg to 100 mg
·Ready-to-Use mRNA-LNPs：150+ pre-validated mRNA sequence

Service Details

Encapsulation Parameters	QC Specifications
Scale：100 μg-100 mg	pH：6.0-8.0
Payload Capacity：100 nt~20 kb mRNA	Size Distribution：40-180 nm
Cationic Lipids：SM102 / ALC-0315	PDI：≤ 0.5
Turnaround Time：5-8 BD	Encapsulation Efficiency：≥ 85%
Shipping Condition：Dry ice, Single freeze/thaw cycle	RNA Content：0.1±0.03 mg/ml
Optional Test Annotation：Zeta、Empty Vesicle Ratio (optional testing require additional charge)

LNP-mediated transfection of mRNA-EGFP in cells

Levostar encapsulated GFP mRNA into LNPs and evaluated delivery efficiency in 293T and A549 cells. The results showed robust GFP expression in both cell types, confirming effective cellular uptake and translation of LNP-formulated mRNA. This demonstrates the potential of LNP-mediated delivery for efficient gene expression across different cell models.

293T Cell

A549 Cell

Fluc mouse fluorescence detection assay

LNP01-1, LNP01-2, LNP01-5, and LNP01-6 were formulated with Fluc mRNA and administered into BALB/c mice. Results confirm that LNP-mediated delivery enables efficient in vivo translation of Fluc mRNA, with distinct formulation-dependent differences in expression levels.

FAQ

What is LNP encapsulation and why is it important for mRNA delivery?

LNP encapsulation is the process of packaging mRNA molecules within lipid nanoparticles to protect them from degradation and facilitate cellular delivery.Efficient encapsulation is critical for ensuring mRNA stability, improving delivery efficiency, and enabling successful protein expression in target cells.

What factors affect LNP encapsulation efficiency?

Encapsulation efficiency is influenced by multiple factors, including lipid composition, mRNA concentration, mixing conditions, and buffer systems.Optimizing these parameters is essential to achieve high loading efficiency, uniform particle size, and consistent performance in downstream applications.

How does microfluidic mixing affect LNP size and encapsulation performance?

Microfluidic mixing enables precise and rapid mixing of lipids and mRNA, resulting in uniform nanoparticle formation with controlled size and low polydispersity.Compared to conventional mixing methods, microfluidics improves reproducibility and encapsulation efficiency, making it a preferred approach for scalable and high-quality LNP production.

What LNP formulations can Levostar provide?

Levostar has more than 15 LNP formulations available, including almost all the published and popular formulations, which can be directly used for research. Levostar also has its own spleen-targeted formulation.

Does Levostar provide LNP encapsulation services for all the catalog mRNAs?

Yes, Levostar provides LNP encapsulation services for more than 200 catalog mRNA, with favorable price. Customized RNA synthesis and LNP encapsulation is also welcome.

Resource

LNP Encapsulation

Learn about Levostar’s end-to-end LNP encapsulation platform, from sequence design and RNA production to scalable encapsulation, nanoparticle characterization, and final product delivery.

LNP Encapsulation

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LNP Encapsulation