MonoPlasmid-Levostar Biotech_Innovative Solutions for Innovative Therapies

Services

Home

monoPlasmid

Levostar Biotech has developed a proprietary “monoPlasmid” platform specifically designed for mRNA plasmids to address the heterogeneity caused by the presence of polyA sequences. Using synthetic biology screening technology, we have identified and integrated DNA sequences that stabilize polyA tails and prevent plasmid polymerization during plasmid passaging.

Our platform consistently delivers plasmids with over 95% supercoiled monomer content, offering a reliable solution to the challenges of multimer formation while maintaining exceptional quality and stability. At the same time, we developed the mRNA-specific strain "xNAboost™," which stabilizes mRNA plasmids and doubles plasmid yield.

mRNA exclusive plasmid platform - monoPlasmid

What is monoPlasmid

pLevoA Plasmid

PolyA Tail Cloning Plasmids

pLevoB Plasmid

PolyA Tail Stabilization Plasmids

pLevoUC Plasmid

One Solution for All

StarK1 Strain

mRNA-specific stable strain

Why monoPlasmid

Enhance mRNA plasmid stability

Maintain polyA tail stability across 20+ passages

Promote high supercoiling ratio in mRNA plasmids

Ensure over 95% monomeric supercoiled content

Boost mRNA plasmid production yield

Reach a yield of up to 500 mg/L.

Case Study:

1.PolyA tail loss issue:

Due to its low free energy, the polyA sequence in plasmids is prone to breakage within E. coli, leading to partial or complete loss of the polyA tail during plasmid repair. Such polyA-deficient plasmids cannot be used to produce functional mRNA. Our monoPlasmid platform incorporates a specialized sequence that significantly reduces the likelihood of such loss.

Sanger Sequencing Results of PolyA Tail

partial loss of the polyA tail in common plasmids

no loss in monoPlasmid

2.Plasmid multimer issue:

Due to the presence of the polyA tail, mRNA plasmids are highly prone to inter-plasmid recombination, resulting in the formation of large plasmids as dimers or multimers. Multimer formation significantly reduces plasmid yield. Our monoPlasmid platform incorporates elements that prevent multimer formation, ensuring a plasmid monomer supercoiling rate of over 95% while also substantially increasing plasmid yield.

common plasmid with multimers

monoPlasmid with high ration monomers

Resource

monoPlasmid

Learn about how our monoPlasmid platform stabilizes polyA-containing mRNA plasmids, reduces multimer formation, and supports high-quality, high-yield plasmid production.

monoPlasmid

File Download

monoPlasmid