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mRNA Synthesis

Levostar Biotech offers a fully integrated mRNA production platform designed to meet the evolving needs of customers at every stage of development. From sequence to mRNA, our end-to-end solutions ensure top-tier quality control throughout the process. Leveraging cutting-edge technologies seamlessly integrated with LNP delivery systems, ensuring top-tier quality control, optimal performance, and continuous innovation at every step.

Service Advantages

Integrated Capabilities

From sequence design to RNA synthesis and LNP formulation—fast delivery in 3 weeks

UTR Selection

Selection of UTRs for tissue specific targeting

Proprietary Plasmid Backbone

Gene synthesis with optimized proprietary IP polyA plasmid

Customized Modifications

Custom 5’ cap and modified NTP options

High-Quality Assurance

Advanced purification and variety of QC options

Service Details

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Sequence Input
	● Length: 200nt~10000nt
	● 5’Cap Options: Uncapped, cap0, cap1
	● Modifications: Unmodified, N1-Me-Ψ, Cy5-UTP, 5moU
Deliverables
	● RNA Types: mRNA, circRNA, saRNA
	● Purification Methods: RNA clean beads, oligo dT purification
	● Quantity: Choose from 100μg to 50mg
Turnaround Time Options	<4000nt	4001-6000nt	>6000nt
	2 weeks	3 weeks	TBD

*To learn more about LNP encapsulation, please click here.

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mRNA Synthesis Request Form

support@levostar.com

Please refer to Quality Controls for more details

	Test Method	Specification	RUO Default	RUO Upgrade	Preclinical Default	Preclinical Upgrade
Appearance	Visual Inspect	Clear and free of foreign particles	+	+	+	+
RNA Content	UV Absorbance	Target ± 5%	+	+	+	+
pH	pH meter	Target ± 0.5	+	+	+	+
A260/280 Ratio	UV Spec	1.70 ~ 2.30	+	+	+	+
Size based purity	Capillary Electrophoresis	≥ 85%	+	+	+	+
Capping Efficiency	LC-MS	≥ 80%	-	+	+	+
poly A Length	LC-MS	Target ± 5%	-	+	+	+
Total protein residue	Nano Orange Assay	≤ 1%	-	-	+	+
Plasmid DNA Residue	qPCR	≤ 0.05%	-	-	-	+
dsRNA	ELISA	≤ 0.1%	-	-	+	+
Sequencing	Sanger Sequencing	>98% Alignment	-	-	-	+
Sequencing	NGS	Q20 Ratio > 90%	-	-	-	Upon Request
Endotoxin	Quantitative	< 10EU/mg	-	-	+	+

One-Stop Solution for Your mRNA

Comparison of different IVT RNA payload formats

	mRNA	Circular RNA	Self-Amplifying RNA
Structure	Linear; Open 5’ and 3’ end mRNA exonuclease degradation	Close loop; No free 5’ 3’ end eliminating mRNA exonuclease degradation	Linear; Open 5’ and 3’ end mRNA exonuclease degradation
Ribosome recruiting	5’ cap	Internal Ribosome Entry Site (IRES)	5’ cap
UTR	Regulatory elements in the 5' and 3' UTR stabilize mRNA and enhance translation initiation	Include IRES + PIE + other elements	Replicon for further self-replication of the mRNA
ORF	Insert < 10 kb	Insert < 3 kb	Insert < 6 kb
PolyA tail	Stabilizes RNA molecule & Prevents degradation	Not required	Stabilizes RNA molecule & Prevents degradation
Immunogenicity	Low	Medium	High
Protein Expression	Strong	Stronger	Strongest
Expression half-life	Short	Longer	Longest
Dosage requirement	High	Medium	Low

Resource

mRNA Synthesis

Learn about our integrated mRNA synthesis platform, from sequence design and plasmid optimization to RNA synthesis, purification, quality control, and LNP formulation support.

mRNA Synthesis

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mRNA Synthesis