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saRNA Synthesis

Self-amplifying RNA (saRNA) is a form of mRNA that encodes a replicase, enabling replication of the original RNA strand within cells. While it shares key structural elements with conventional mRNA—such as a linear, single-stranded structure with a 5′ cap, 3′ polyA tail, and untranslated regions (UTRs)—saRNA stands out for its ability to achieve lower dosages, reduce side effects, and provide longer-lasting vaccine efficacy.
Levostar offers custom self-amplifying RNA (saRNA) synthesis with tailored modifications and reliable quality to accelerate your innovative research.

Service Details

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Sequence Input
	● Length: 200nt~6000nt
	● 5’Cap Options: Uncapped, Cap0, Cap1
	● Modifications: Unmodified, m5c
Deliverables
	● Purification Methods: RNA Clean Beads, Oligo dT purification
	● Quantity: Choose from 10μg to 1mg
Quality Control
	● Identification: 100% sequence accuracy for CDS regions
	● Purity: ≥ 70% size-based purity by Capillary Electrophoresis
Turnaround Time Options	<2000nt	2001-4000nt	4001-6000nt
	3 weeks	4 weeks	5 weeks

*To learn more about LNP encapsulation, please click here.

Comparison of different IVT RNA payload formats

	mRNA	Circular RNA	Self-Amplifying RNA
Structure	Linear; Open 5’ and 3’ end mRNA exonuclease degradation	Close loop; No free 5’ 3’ end Eliminating mRNA exonuclease degradation	Linear; Open 5’ and 3’ end mRNA exonuclease degradation
Ribosome recruiting	5’ Cap	Internal Ribosome Entry Site (IRES)	5’ Cap
UTR	Regulatory elements in the 5' and 3' UTR stabilize mRNA and enhance translation initiation	Include IRES + PIE + other elements	Replicon for further self-replication of the mRNA
ORF	Insert < 10 kb	Insert < 3 kb	Insert < 6 kb
polyA tail	Stabilizes RNA molecule & Prevents degradation	Not required	Stabilizes RNA molecule & Prevents degradation
Immunogenicity	Low	Medium	High
Protein Expression	Strong	Stronger	Strongest
Expression half-life	Short	Longer	Longest
Dosage requirement	High	Medium	Low