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RNA Transfection Kit

RNA transfer kits are essential tools for efficient delivery of RNA molecules into cells, enabling gene expression, gene silencing, and functional studies across diverse biological systems. They are widely applied in areas such as molecular biology, immunology, oncology, and in vivo therapeutic research.

Levostar RNA transfer kits provide powerful solutions for delivering RNA with high efficiency and reliability. Covering standard cell transfection, immune cell transfection, and in vivo applications, our portfolio supports a wide range of research needs—from basic cell biology to immunotherapy and preclinical studies. With optimized formulations for different systems, Levostar enables researchers to unlock RNA’s full potential and accelerate breakthroughs in discovery and therapeutic innovation.

Common cell RNA transfection kit
Immune cell transfection kit
In vivo RNA transfection kit

Product Description

1. Common Cell RNA Transfection Kit

The Common Cell RNA Transfection Kit (SDR8006) is designed for common cell lines, offering an efficient and reliable transfection solution that simplifies and accelerates RNA research in these cells.

Product Details

Product Manual

Cat.#	Specification	Product Composition
Cat.#	Specification	Transfection Reagent	Buffer
SDR8006	1.5mL	1.5mL × 1 vial	8mL × 1 vial
	3.0mL	3.0mL × 1 vial	8mL × 2 vials
	5.0mL	5.0mL × 1 vial	8mL × 3 vials

Product Advantages

▪ High Cell Expression: Through unique transmembrane transport and endosomal escape mechanisms, easily achieving transfection efficiencies above 90%.

▪ Compatible with Various RNA Types: Suitable for transfecting mRNA, siRNA, gRNA, saRNA, circRNA, and other RNA types.

▪ Extremely Low Cytotoxicity: Utilizes biodegradable materials, free of ethanol, with minimal impact on cells, reducing experimental bias.

▪ Broad Cell Compatibility: Successfully validated in over 100 cell types, including A549, Capan-2, and HEK293, with most achieving transfection efficiencies exceeding 90%. SDR8006 far surpasses industry standards.

Experimental Results

1. Messenger RNA (mRNA)

Using a 24-well plate, transfect with eGFP mRNA, followed by fluorescence imaging and flow cytometry analysis after 24 hours.

2. Small Interfering RNA (siRNA) - Cellular Uptake Efficiency

Transfect with 30nM Cy3-labeled siRNA per well, followed by fluorescence imaging after 6 hours.

3. Circular RNA (circRNA)

Using a 96-well plate, transfect with 100ng eGFP circRNA per well, followed by fluorescence imaging after 24 hours.

4. Self-Amplifying RNA (saRNA)

Using a 96-well plate, transfect with 100ng eGFP saRNA per well, followed by fluorescence imaging after 24 hours.

2. Immune Cell Transfection Kit

The Immune Cell RNA Transfection Kits are specially developed for hard-to-transfect immune cells, including T cells, B cells, dendritic cells, and other primary immune populations. With optimized formulations, these kits provide high efficiency and low cytotoxicity, enabling reliable RNA delivery without compromising cell viability or function. They simplify complex immune cell manipulation and accelerate research in areas such as immunotherapy, vaccine development, and immune response studies.

Product Details

Product Manual

Catalog Number	Product Specification	Product Composition
Catalog Number	Product Specification	Transfection Reagent	Buffer	Enhancer
SDR2008	SDR2008-1.5mL	1.5mL × 1 vial	8mL × 1 vial	70µg × 1 vial
SDR2008	SDR2008-3.0mL	3.0mL × 1 vial	8mL × 1 vial	10µg × 2 vial
SDR2006	SDR2006-1.5mL	1.5mL × 1 vial	8mL × 1 vial	/
	SDR2006-3.0mL	3.0mL × 1 vial	8mL × 1 vial	/
	SDR2006-5.0mL	5.0mL × 1 vial	8mL × 1 vial	/

Product Advantages

▪ Efficient: Achieves high transfection efficiency in both immune cell lines and primary immune cells, while maintaining excellent cell viability.

▪ Flexible: Compatible with a wide range of immune cell types, including T cells, B cells, dendritic cells, and other primary immune populations.

▪ User-Friendly: Simple and rapid workflow with optimized formulations, reducing experimental complexity and saving valuable time.

Experimental Results

· Human Primary T Cell Transfection

Using a 24-well plate, transfect with 1µg eGFP mRNA per well, followed by fluorescence microscopy and flow cytometry analysis after 24 hours.

· Human Primary T Cell Transfection

Jurkat Cell Line: Using a 24-well plate, transfect with 1µg eGFP mRNA per well, followed by fluorescence imaging and flow cytometry analysis after 24 hours.

· Other Cell Line

Using a 24-well plate, transfect with 500ng eGFP mRNA per well, followed by fluorescence imaging and flow cytometry analysis after 24 hours.

3. In vivo RNA Transfection Kit

The In vivo RNA Transfection Kit (SDR8002) is a versatile in vivo RNA delivery solution. Utilizing innovative ready-to-use LNP in vivo delivery technology, SDR8002 supports transient encapsulation of siRNA, miRNA, sgRNA, mRNA, and other RNA types, enabling systemic RNA transfection via multiple administration routes, providing a more efficient and rapid solution for RNA research and clinical translation.

Product Details

Product Manual

Cat.#	Specification	Product Composition
Cat.#	Specification	Transfection Reagent	Buffer	RNA Positive Control
SDR8002/ SDR8003/ SDR6005	0.3mL	0.3mL × 1 vial	8mL × 1 vial	10µg × 1 vial
	0.5mL	0.5mL × 1 vial	8mL × 2 vials	10µg × 1 vial
	1.0mL	1.0mL × 1 vial	8mL × 3 vials	10µg × 1 vial

Product Advantages

▪ Efficient: Maintains stable high transfection levels regardless of injection method.

▪ Flexible: Customizable for systemic or localized RNA delivery based on research goals.

▪ User-Friendly: Optimized delivery solutions enable rapid, efficient RNA encapsulation, simplifying experiments.

Experimental Results

1. Intravenous Injection (IV) - Targeting Liver/Spleen

2. Intramuscular Injection (IM) - Vaccines

3. Intraperitoneal Injection (IP) - Multi-Organ/Tissue RNA Delivery

Resource

Ready-to-use RNA LNP Kits

Learn about our RNA transfection kits for efficient delivery of mRNA, siRNA, circRNA, saRNA, and other RNA molecules in cell-based and in vivo research applications.

Ready-to-use RNA LNP Kits

File Download

Ready-to-use RNA LNP Kits