Plasmid Preparation-Levostar Biotech_Innovative Solutions for Innovative Therapies

Plasmid Preparation

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Levostar’s plasmid preparation service provides high-quality plasmids at scales from micrograms to grams. We emphasize speed, flexibility, and DNA quality, ensuring superior results for your specific needs. Our services accommodate both small research labs and large biotech and pharmaceutical companies, delivering 100% sequence accuracy and guaranteed yields. We ensure >90% supercoiled ratio to guarantee the transfection efficiency of your plasmids. At the same time, with robust QC options and extensive experience in downstream applications such as mRNA, lentivirus, and AAV production, we provide tailored solutions for complex sequences, including extended polyA tails and ITRs.

"pDNAboost" is a preparation solution designed specifically for low-copy plasmids, capable of significantly enhancing the yield and purity of low-copy plasmid extractions.

GMP-like Manufacturing

From micrograms to grams

Ultra-Low Endotoxin

Reduced to as low as 5 EU/mg

High Supercoiled Content

Over 90% supercoiled plasmid purity

Animal- and Antibiotic-Free

Without animal-derived components or antibiotic resistance genes

GMP-like Manufacturing

A faster, cost-effective alternative to full cGMP standards

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	Research Level	High Quality Grade	GMP-Like Grade
Application	For all-purpose use	Optimized for transfection and animal studies	Optimized for pre-clinical and early phase trials
Scale	≥ 5μg	≥100μg	≥50mg
Endotoxin Level	not guarantee ≤100EU/mg	≤100EU/mg ≤10EU/mg ≤5EU/mg	≤10EU/mg ≤5EU/mg
Homogeneity	Predominantly supercoil	≥80% supercoil ≥90% supercoil	≥90% supercoil
Antibiotics-free Production	NA	NA	Option
Animal-free Production	NA	Option	Option
Document	Certificate of analysis	Certificate of analysis	Certificate of analysis DNA Preparation Report

Plasmid Preparation Request Form

support@levostar.com

QC Test	Specification	Research Level	High Quality Grade	GMP-Like Grade
Appearance	Clear, colorless, and free from precipitates or foreign particles	+	+	+
DNA Content	Depends on actual yield	+	+	+
A260/230; A260/280	A260/230 ≥2 ; A260/280: 1.8-2.0	+	+	+
Restriction Analysis	Agarose gel electrophoresis	+	+	+
Sequence Accuracy	Consistent with reference sequence	+	+	+
Endotoxin Level	Depends on your choice	+	+	+
Homogeneity	Agarose gel electrophoresis	Predominantly supercoil	≥80% supercoil	≥90% supercoil
Homogeneity	HPLC	Predominantly supercoil	≥80% supercoil	≥90% supercoil
Bio-Burden Test	No viable microorganisms in/on culture media after 72hrs	-	+	+
Residual RNA	Not visible on agarose gel	+	+	+
Residual RNA	≤1% by qPCR	-	√	+
Residual RNA	< 15% by agarose gel electrophoresis	+	+	-
E.coli Genomic DNA	≤1% by qPCR	-	√	+
Total Protein	≤ 1% by NanoOrange assay	-	√	+
Animal-free Production	TSE/BSE statement	√	√	√
Whole Plasmid Sequencing	Sanger/NGS/Nanopore sequencing	√	√	√
polyA Guarantee	Polyadenylation purity and length guaranteed	√	√	√
AAV ITR Guarantee	ITR sequence integrity guaranteed	√	√	√

Notes:

1. “+” means a mandatory test; “-” means a non-mandatory test; “√” means an optional test available with an additional charge.

2. Sequence verification is performed only within the insert region. Whole plasmid sequencing service is available with an additional charge.

Commercial

Research Level

High Quality Grade

GMP-Like Grade

cGMP Grade

Plasmid Preparation

Learn about our reliable plasmid preparation services, from plasmid amplification and purification to endotoxin control, concentration adjustment, quality testing, and delivery of ready-to-use plasmid DNA for downstream research applications.

Plasmid Preparation

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Plasmid Preparation