mRNA-eGFP-Firefly Luciferase

Cat.#	Gene	Scale	State
LS-RM05	mRNA-eGFP-Firefly Luciferase	1mg	Lyophilized/Liquid

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Description
Product Overview
Sequence
Data Chart
Storage & Logistic
Documents

Cat.# Gene Scale State

LS-RM05 mRNA-eGFP-Firefly Luciferase 1mg Lyophilized/Liquid
Product Overview

The mRNA-eGFP-Firefly Luciferase is a ready-to-use mRNA product that combines enhanced green fluorescent protein (eGFP) with Firefly Luciferase (Fluc). The open reading frame (ORF) of eGFP is derived from the jellyfish Aequorea victoria and emits green fluorescence (507 nm) upon excitation at 488 nm, making it suitable for real-time fluorescent microscopy imaging. The Fluc gene, originating from the firefly Photinus pyralis, encodes an enzyme that catalyzes the oxidation of the substrate D-luciferin to generate a bioluminescent signal with an emission wavelength of approximately 560 nm.

Product Details

Storage Buffer: ddH₂O ph=7.0

mRNA length: 2673 nt

Amino acid sequence size: 791 aa

Handling and Storage: store at - 80 ℃ for long term
Amino acid sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSSMEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV
Cell Expression Result

Cell Line: 293T

Culture Plate: 96-well plate

Cell Confluency: 80%

Transfection Dose: 200 ng per well
Storage & Logistic

Storage Conditions: 2°C to 8°C, protect from light

Shipping Conditions: Ambient

Expiration Date of Liquid Product: 12 Months

Retest Date of Powder Product: 18 Months