- Description
- Product Overview
- Sequence
- Data Chart
- Storage & Logistic
- Documents
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Cat.# Gene Scale State LS-RM030 mRNA-Gaussia Luciferase 1mg Lyophilized/Liquid -
Product Overview
mRNA-Gaussia Luciferase (mRNA-GLuc) is a ready-to-use mRNA product encoding Gaussia luciferase (GLuc). It is suitable for in vitro transcription (IVT) assays, mRNA delivery studies, reporter gene detection, in vivo monitoring of secreted luciferase, and high-throughput screening experiments. GLuc is a highly sensitive secreted luciferase that enables real-time, non-lytic bioluminescence analysis by measuring luciferase activity in culture media or biological fluids, making it ideal for both in vitro and in vivo applications.
Product Details
Storage Buffer: ddH2O ph=7.0
mRNA length: 855 nt
Amino acid sequence size: 185 aa
Handling and Storage: store at - 80 ℃ for long term
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Amino acid sequence
MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKELEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPLEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGD
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Cell Expression Result
Cell Line: 293T Culture
Plate: 96-well plate
Cell Confluency: 80%
Transfection Dose: 200 ng per well
Result(The assayed sample is cell culture supernatant)
Name
Lysis Buffer(μL)
Dilution Factor
Volume Tested(μL)
Luciferase Assay Substrate(μL)
Result
mRNA-Gaussia Luciferase
100
10
1
100
109,100
Negative
N/A
N/A
1
100
173
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Storage & Logistic
Storage Conditions: 2°C to 8°C, protect from light
Shipping Conditions: Ambient
Expiration Date of Liquid Product: 12 Months
Retest Date of Powder Product: 18 Months
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